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Service

This section provides basic information about Service

aCGH Analysis

Human Genome CGH Microarrays

The Agilent Human Genome CGH Microarray is a dual color array containing 60-mer oligonucleotide probes synthesized in situ using Agilent’s inkjet SurePrint technology. Its comprehensive probe coverage allows genome-wide DNA copy number variation profiling while focusing on known genes, promoters, miRNAs, pseudoautosomal and telomeric regions.

  • Multiple microarray formats and bundled options available
  • Distinct microarray catalog kits containing regions recommended by ISCA
  • Probes annotated against NCBI Build 37 (UCSC hg19, February 2009)

Comparative genomic hybridization (CGH) is used for genomic copy-number variations (CNV) research. CGH microarray technology platform by Agilent, Inc. allows for the discovery of novel oncogene, anti-oncogene, drug target, and biomarkers through identifying chromosomal abnormalities in cancer and genetic disorders.

Comparison of cytogenetic techniques for identifying chromosomal abnormalities.

Types of Agilent Arrays

Many variations of array exist based on the resolution. Not only human, but also arrays for mouse, rat, and chicken are available, and others besides them are also customizable. If you provide us with DNA of high quality, you are guaranteed to receive the finest result possible, and please note that different DNA amount may be required based on the type of chips

  • Slide Design based on Different Formats

Example for workflow & requirement

Agilent SurePrint G3 CGH Microarrays

  • Benefit
  • CGH microarray technology platform from Agilent makes it possible to discover novel genes associated with cancer, anti-oncogenes, drug targets, and biomarkers by investigating various chromosome abnormalities in hereditary disorders. There are various types, depending on the resolution, and there are also products for not only human, but also mouse, rat, and chicken. It can also be customized according to customers’ needs

  • Workflow
  • Sample requirement
  • 1x1M : 3ug
    2X400K : 1.5ug
    4X180K : 1.5ug
    8X60K : 500ng
    DNA Qualification: 260/280 1.8~2.0, 260/230 ratio of over 1.5, (by gel image) no RNA, no degradation

  • Turnaround timew
  • DNA QC: 2~3 days
    3~4 days for 4 arrays, regardless of array type



Products

  • SurePrint G3 Human CGH Microarray Kit, 1x1M
  • SurePrint G3 Human CGH Microarray Kit, 2x400K
  • SurePrint G3 Human CGH Microarray Kit, 4x180K
  • SurePrint G3 Human CGH Microarray Kit, 8x60K
  • SurePrint G3 Unrestricted CGH ISCA v2, 4x180K
  • SurePrint G3 Unrestricted CGH ISCA v2, 8x60K
  • Human Genome CGH Microarray 244A Supplemental
  • Human Genome CGH Microarray Kit 105A
  • Unrestricted HD-CGH Microarray ISCA v2, 2x105K
  • Unrestricted HD-CGH Microarray ISCA v2, 4x44k
  • Human Genome CGH Microarray Kit 244A
  • Human Genome CGH Microarray Kit 244A
  • Human Genome CGH Microarray Kit, 4x44K
  • SurePrint G3 CGH ISCA v2 Microarray Kit, 8x60K

Bioinformatics Service

  • Basic analysis
    • CNV analysis software: Agilent Genomic Workbench
    • Algorithm: ADM-1, ADM-2, CBS
    • Aberration summary report ( PDF format file )
    • Aberration report ( EXCEL format file )
  • Advanced analysis
    • Upon request, an advanced analysis is processed through a professional consultation with an extra fee charged.