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Service

This section provides basic information about Service

Exome Sequencing

Overview

Exome Sequencing is a method that selectively analyzes only coding region (Exon), which composes 1-2% of the genome. It is a cheaper and effective way of sequencing compared to whole genome sequencing that analyzes the entire genome. Not only does it sequence single nucleotide polymorphism (SNP), but exome sequencing also identifies insertion and deletion. Based on these analysis results, exome sequencing can be effectively used in research for rare disease, cancer genomics, and genetic disorders.


Exome Capture method

DNA Link uses various Exome Capture kits to provide Exome sequencing service.

Agilent SureSelect
illumina Nextera Rapid (Expanded)
Ion AmpliSeq Exom
Method
Solution Hybridization
Hybridization
Targeted PCR
Target size
50M~75M(V3~V5+UTR)
37M (62M)
33M(~58M)
Contents
Exons(+UTR)
Exons(Exons, UTR, miRNA)
Exons
Library Design Time
3days
1.5days
6hrs
Input DNA
1ug (3ug)
50ng
50~100ng
Sequencer
Hiseq 2000/2500
Ion Proton system

Main Capture Products

  • SureSelect Human All Exon Kits (for the HiSeq Sequencer)
  • SureSelect Human All Exon V5 (targeting coding regions only) and V5+UTRs (targeting coding regions plus UTRs)
  • SureSelectXT Human All Exon 50Mb
  • SureSelectXT Human All Exon V4
  • SureSelectXT Human All Exon V4+UTRs
  • SureSelectXT Human All Exon V5
  • SureSelectXT Human All Exon V5+UTRs
  • SureSelectXT Human All Exon V5+lncRNA
  • SureSelectXT Human All Exon V5 Plus
  • Ion torrent Exome Kits (for the Ion Proton™ System)
  • Ion AmpliSeq™ Exome Kit
  • Ion TargetSeq™ Exome Enrichment : Ion-TargetSeq-Exome-50Mb-hg19

Experiment Work Flow

platform
protocol
Sample Type
minimum Concentration
minimum volume
Required Amount (including QC)
HiSeq
SureSelect exome
gDNA
25ng/ul(3ug) or 8.4ng/ul(1ug)
120ul
4ug or 2ug
HiSeq
Truseq exome
gDNA
20ng/ul
55ul
2ug
HiSeq
Nextera Rapid Capture Exome
gDNA
5ng/ul
10ul
500ng
HiSeq
Nextera Rapid Capture Expanded Exome
gDNA
5ng/ul
10ul
500ng
Proton
SureSelect(modify)
gDNA
8.4 ng/μL
120ul
2ug
Proton
LT exome
gDNA
8.4 ng/μL
120ul
2ug
Proton
AmpliSeq Exome
gDNA
4.8ng/ul
21ul
1ug

Turnaround time

platform
Illumina HiSeq Agilent SureSelect
Ion ProtonTM IonAmpliSeq Exome
Run Info
2x100 Paird End
Ion PITM Chip(v2)
Time for Experiment * Based on production of raw data
6 weeks
2 weeks
* The time is based on the production of raw data, and may be adjusted depending on the number of samples.