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Service

This section provides basic information about Service

mRNA Sequencing

Overview

The recent development of Next Generation Sequencing technology has allowed the production of bulk sequencing data. Whole transcriptome analysis using these bulk data not only provides the expression level between genes, but also provides the data of novel transcriptome, discovery of isoform, and fusion gene.

RNA Sequencing Summary

Application
Method
Feature
Sequencing Platform
mRNA
Selection using poly‐T oligo
Strand specific


Hiseq 2000/2500&Ion ProtonTM
Total RNA
rRNA Depletion
Strand specific mRNA and various non coding RNA (lincRNA,snRNA, snoRNA) analysis
Small RNA
Size selection(20~70bp)
Separate analysis of non coding RNA and micro RNA

Benefit

RNA-seq vs Microarray
RNA-SEQ
Microarray
Production of reproducible data
O
O
Dynamic range
105
102
Background signal
Low
High
Alternative splice site check
O
X
Discovery of novel Isoform (Fusion)
O
X
Analysis of sample with no standard genomic information
O
X

Workflow

Sample requirements

Hiseq
Proton
Quality
RIN ≥ 8 , No DNA contamination
RIN >7, high-quality, intact RNA
Concentration
≥100ng/ul
≥100ng/ul or ≥625ng/ul
Amount
Total RNA 2ug
RNA 6ug

Turnaround time

Hiseq : within 4 weeks
Ion Proton : within 3 weeks