|RNA-Seq type||Details||Price||Deliverable||Reference Code|
|Whole transcriptome sequencing||mRNA-Seq by Poly(A) capture||$ 299
||20 - 30M reads, 75bp||WTRA|
|Whole transcriptome sequencing||mRNA-Seq by ribo-depletion||$ 399
||20 - 30M reads, 75bp||WTRR|
|Expression Profiling Sequencing||3’ end sequencing||$ 200
||10M reads, 75bp||EPS3|
|Full-length RNA-Seq (Iso-Seq)||Iso-Seq (PacBio Sequel SMRT)||$ 2,400
||5Gb long reads||ISOQ|
|Small RNA-Seq (SMRQ)||TruSeq (illumina)||$ 349
||10 - 20M reads||SMRQ|
* Minimum number of sample requirement may apply.
**Promotional price lasts until May 2017
*** Price includes library preparation, sequencing, and raw data delivery, not data analysis.
Whole Transcriptome Sequencing
Whole transcriptome sequencing is a major advance in the study of gene expression compared to traditional microarray-based approaches in that it provides a comprehensive view of a cellular transcriptional profile at a given biological moment enabling not only qualitative but also quantitative analysis of the transcriptome. It also provides information about diverse variations occurring at RNA level of post-transcriptional modifications such as splice variants and isoforms.
Expression Profiling Sequencing
Taken apart the quantitative aspect of whole transcriptome sequencing, expression profiling sequencing gives most comprehensive insight about the transcription level of individual mRNAs which is then further related to the expression level of proteins. It is a very cost-effective way of understanding the transcriptions in that it only sequences a small part of mRNA (normally 3’ end with poly (A) tail) and counts the number of sequences. It provides virtually unlimited dynamic range for highly abundant transcripts as well as not missing rare transcripts.
Empowered by the unique advantage of long reads from PacBio systems, isoform sequencing (Iso-Seq) reveals the splice variants of the transcripts providing the most comprehensive landscape of a transcriptome. The splicing junction of mRNA can be pin-pointed and this can be aligned back to the genome data to show what kind of splice variants are produced, further giving insights about the relation between splicing and a certain phenotype or diseases.
Small RNA Sequencing
Small, non-coding RNA, or MicroRNAs are a short, 18-22 bp nucleotides which is known to play a critical role in the gene regulation and expression.
DNA Link provides diverse options of flowcell / lane rental as well for the researchers who have their own sequencing library. Any libraries, for example, ChIP-Seq, ATAC-Seq, and so on can be run in our sequencers to ensure the least cost and fast turnaround time for the researchers.
|Platform||Run mode (high-throughput mode)||Price||Deliverable||Product Code|
|illumina HiSeq4000||50SR, per lane||$ 1,850
||single reads from approx. 300M clusters||HSF50SR|
|illumina HiSeq2500||100SR, per lane||$ 2,450
||single reads from approx. 225M clusters||HST100SR|
|100PE, per lane||$ 2,950
||paired reads from approx. 225M clusters||HST100PE|
|illumina NextSeq500||75SR, per flowcell||$ 2,450
||single reads from approx. 400M clusters||NSF75SR|
|75PE, per flowcell||$ 4,750
||paired reads from approx. 400M clusters||NSF75PE|
|150PE, per flowcell||$ 7,750
||paired reads from approx. 400M clusters||NSF150PE|
|Pacific Biosciences Sequel||1 SMRT cell||$ 1,950||4-6Gb of long reads||SQSMRT|
|Pacific Biosciences RSII||1 SMRT cell||$ 750||0.8Gb of long reads||RSSMRT|
DNA Link Sequencing Lab is a sequencing service provider which has been working in the industry for more than a decade. It has official accreditation from illumina, PacBio, Thermo fisher and Affymetrix, as well as ISO9001:2015 certification. With its specialty in long-read single molecule sequencing as well as RNA-Seq and diverse applications, it is providing genetic services to thousands of researchers in the world.
Our fleet of sequencers include both second and third generation platforms including HiSeq and NextSeq from illumina , Sequel and RSII from Pacific Biosciences, and ion torrent and S5 from Thermo Fisher Scientific.