image

Service

This section provides basic information about Service

Small RNA Sequencing

Overview

Small RNA Sequencing analyzes only Small RNA using NGS platform.

Query thousands of small RNA sequences with unprecedented sensitivity and dynamic range for both small RNA discovery and profiling applications. Find novel microRNAs, characterize variation such as isomiRs with single-base resolution, and analyze the differential expression of all small RNAs in any sample without prior assumptions.

Benefit

- Due to the wide dynamic range of detection, it works for all small RNA.
- Not just known miRNA, but it can detect novel miRNA and make a prediction.
- Universal Platform
- Customizable Size Selection
- Simple Workflow

Workflow

Sample requirements

Hiseq
Proton/PGM
Quality
RIN ≥8, No DNA contamination
RIN >6, high quality total RNA
Concentration
≥200ng/ul
small RNA≥334ng/ul or total RNA≥67ng/ul
Amount
Total RNA 2ug
small RNA 2ug or total RNA 6ug

Turnaround time

Hiseq : within 4 weeks
Ion Proton : within 3 weeks