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Service

This section provides basic information about Service

Targeted Resequencing

Overview

Target Resequencing analyzes sequence of only the targeted region from the entire genomic DNA. The method is universally used in analyzing data of Genome Wide Association Study (GWAS) and selectively obtaining genomic region of specific disease. Methods such as, Capture, long range PCR, and multiplex PCR are often used in securing sequence of target region, and appropriate method should be selected based on the number of samples and the size of target region.

Benefit

Able to design probe or primer pool for the region of interest as a way of capturing selected target.
- Provides capture service from various platforms of NimbleGen, Agilent, and Ion torrent.
- Able to provide customized service by using various NGS Sequencer of HiSeq, 454, PGM, Ion Proton, and PacBio.

WorkFlow(example)

GWAS->Discovery of meaningful DNA -> Selection of target region -> Design of Capture probe and Primer Pool

Turnaround time

HiSeq : 3 weeks
454 : 3 weeks
Ion Proton, PGM : Ampliseq < 10 sample : 2 weeks, Ampliseq ≥ 10 sample : 3 weeks
Pacbio : 2 weeks

Sample requirement

platform
protocol
Sample Type
minimum Concentration
minimum volume
Required Amount (including QC)
HiSeq
SureSelect targeted
gDNA
25ng/ul(3ug) or 8.4ng/ul(1ug)
120ul
4ug or 2ug
HiSeq
Nextera Rapid Capture Custom Enrichment
gDNA
5ng/ul
10ul
500ng
HiSeq
TruSight Cancer
gDNA
5ng/ul
10ul
500ng
HiSeq
TruSight Cardiomyopathy
gDNA
5ng/ul
10ul
500ng
Hiseq
TruSight Inherited Disease
gDNA
5ng/μL
10ul
500ng
HiSeq
TruSight Autism
gDNA
5ng/ul
10ul
500ng
454
Amplicon(fusion primer)
gDNA
10ng/uL
100ul
1ug
454
Amplicon(library prep)
amplicon
32ng/uL
16ul
1ug
Proton
SureSelect(targeted)
gDNA
84ng/ul
120ul
2ug
Proton
LT targeted
gDNA
84ng/ul
120ul
2ug
Proton
Fragment library(amplicon)
gDNA or amplicon
3.4 ng/ul or 6ng/ul
6ul or 50ul
500ng
Proton
amplicon(fusion primer)
amplicon
12.5 ng/ul
4ul
500ng
Proton
cancer panel v2
gDNA
1.7 ng/μL
6ul
500ng
Proton
CCP(Comprehensive cancer panel)
gDNA
17ng/ul
6ul
500ng
Proton
IDP(Inheritied Disease panel)
gDNA
17ng/ul
6ul
500ng
PGM
Fragment library(amplicon)
gDNA or amplicon
3.4 ng/ul or 6ng/ul
6ul or 50ul
500ng
PGM
Fragment library(amplicon)<350bp
gDNA or amplicon
12.5ng/ul or 2.9ng/ul
4ul or 35ul
500ng
PGM
Fragment library(amplicon)>400bp
gDNA or amplicon
12.5ng/ul or 6ng/ul
4ul or 50ul
500ng
PGM
amplicon(fusion primer)
gDNA
12.5ng/ul
4ul
500ng
PGM
cancer panel v2
gDNA
1.7ng
6ul
500ng
Pacbio
amplicon (250bp~2kb)
amplicon
50ng/ul
21ul
3ug
Pacbio
amplicon (5kb~10kb)
amplicon
200ng/ul
30ul
10ug