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Service

This section provides basic information about Service

Bioinformatics

Overview

NGS 기반 Genomics 연구분야는 전문적인 Bioinformatics 지원이 필수적인 요소가 되었습니다. DNA Link 의 Bioinformatics 서비스는 연구자 분들이 프로젝트 수행과정에서 어려움을 격을 수 있는 Bio-IT, 통계, 데이터마이닝 분야에 도움을 드릴 수 있도록 구성되어 있습니다. Standard service, Advanced service 로 나누어져 있으며, 연구자분들의 필요에 따라 customized 서비스 이용도 가능하여 비용절감과 효과적인 연구진행에 기여할 수 있습니다.

저희 DNA Link 는 창사이례 수십건 이상의 정부프로젝트 참여하고 있으며, 논문발표를 통해 Genomics 분야에서의 Bioinformatics 기술력을 인정받고 있습니다. 또한, 국내 최초로 EMC isilon NAS Storage 1.5PB 를 도입하여 차세대 시퀀싱 인프라 자동화체계를 구축하였으며, 1000 cpu core 의 분석서버와 함께 최적의 서비스환경을 보유하고 있습니다.

Service Type
Basic analysis
Advanced analysis
Exome Capture Sequencing
  • Raw data delivery using downloadable link or hard drive devices
  • Analysis using GATK(2.7) best practices guideline
  • Alignment to reference sequence(hg19/b37.2) using BWA(.bam)
  • SNP and short INDEL will be hard filtered to GATK recommendations(.vcf)
  • Sequencing Summary(.xlsx)
  • Alignment Summary(.xlsx)
  • Depth Coverage Plot(.xlsx)
  • Genotype Calling and SNP detection
  • Germline & Somatic Variant Summary and Annotation(.xlsx)
  • Prediction Amino acid substitution and risk score(SIFT, PolyPhen, Phylop, mutationTaster)
  • Frequency estimation using 1000genome and ESP4500
  • NGS-GWAS Analysis
  • Rare Variants Analysis
  • Gene Cluster Annotation(GSEA, KEGG pathway)
  • Customized Analysis(Client Support)
Whole Genome Sequencing
  • Analysis using GATK(2.7) best practices guideline
  • Sequencing Summary
  • Alignment Summary
  • Depth Coverage Plot
  • Genotype Calling and SNP detection
  • Germline & Somatic Variant Summary and Annotation
  • Prediction Amino acid substitution and risk score(SIFT, PolyPhen, Phylop, mutationTaster)
  • Frequency estimation using 1000genome and ESP4500
  • NGS-GWAS Analysis
  • Rare Variants Analysis
  • Gene Cluster Annotation(GSEA, KEGG pathway)
  • Fusion gene analysis
  • CNV analysis
  • Customized Analysis(Client Support)
Target Region Capture Sequencing
  • Analysis using GATK(2.7) best practices guideline
  • Sequencing Summary
  • Alignment Summary
  • Depth Coverage Plot
  • Genotype Calling and SNP detection
  • Variant Summary and Annotation
  • Prediction Amino acid substitution and risk score(SIFT, PolyPhen, Phylop, mutationTaster)
  • Frequency estimation using 1000genome and ESP4500
  • NGS-GWAS Analysis
  • Rare Variants Analysis
  • Gene Cluster Annotation(GSEA, KEGG pathway)
  • Customized Analysis(Client Support)
De-novo Assembly
  • Sequencing Summary
  • Pre-Assemble ( Error correction )
  • De novo Assembly ( contig, scaffold )
  • Consensus Polishing
  • Gene prediction ( need RNA-seq data for Eukaryotes )
  • Genome plot
  • De novo Assembly (contig, scaffold)
  • Gene Annotation (Gene Ontology)
  • Customized Analysis (Client Support)
mRNA Sequencing
  • Sequencing Summary
  • Alignment Summary
  • Summarizing plots
  • Density plot, Box plot, GSEA, Scatter plot, Volcano plot
  • List of genes expressed differentially between given classes
  • List of transcripts expressed differentially between given classes
  • Pathway analysis using DAVID
  • Fusion gene analysis
  • Customized Analysis
smallRNA Sequencing
  • Sequencing Summary
  • Alignment Summary
  • Visualization plot
  • Density plot, Box plot, GSEA, Scatter plot, Volcano plot
  • Targetscan Analysis (limited to specific species)
  • Pathway Analysis
  • Customized Analysis
ChIP Sequencing
  • Sequencing Summary
  • Alignment Summary
  • List of peak positions identified by ChIP-seq tools
  • Input data is transformed into applicable bam or wig format, as needed for visualization by commonly used genome browsers (e.g. IGV or UCSC Genome Browser)
  • Medip analysis
  • Peak analyzer analysis
  • Customized Analysis(Client Support)