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Service

This section provides basic information about Service

Exome Sequencing

Overview

Exome Sequencing 은 유전체상에서 1~2%에 해당하는 coding region(Exon)만을 선택적으로 분석하는 방법으로 전체유전체의 염기서열을 분석하는 whole genome Sequencing에 비하여 저렴하면서도 효과적인 방법입니다. Exome Sequencing을 통해 유전체상에 나타난 Single nucleotide polymorphism(SNP)뿐만 아니라 insertion, deletion 분석까지도 가능합니다. 이러한 분석 결과를 토대로 rare disease, cancer genomics, 질환 유전체 연구에 활용할 수 있습니다



Exome Capture method

DNA Link에서는 다양한 Exome Capture kit을 이용해서 Exome sequencing service를 제공하고 있습니다.

Agilent SureSelect
illumina Nextera Rapid (Expanded)
Ion AmpliSeq Exom
Method
Solution Hybridization
Hybridization
Targeted PCR
Target size
50M~75M(V3~V5+UTR)
37M (62M)
33M(~58M)
Contents
Exons(+UTR)
Exons(Exons, UTR, miRNA)
Exons
Library Design Time
3days
1.5days
6hrs
Input DNA
1ug (3ug)
50ng
50~100ng
Sequencer
Hiseq 2000/2500
Ion Proton system

주요 Capture 제품

  • SureSelect Human All Exon Kits (for the HiSeq Sequencer)
  • SureSelect Human All Exon V5 (targeting coding regions only) and V5+UTRs (targeting coding regions plus UTRs)
  • SureSelectXT Human All Exon 50Mb
  • SureSelectXT Human All Exon V4
  • SureSelectXT Human All Exon V4+UTRs
  • SureSelectXT Human All Exon V5
  • SureSelectXT Human All Exon V5+UTRs
  • SureSelectXT Human All Exon V5+lncRNA
  • SureSelectXT Human All Exon V5 Plus
  • Ion torrent Exome Kits (for the Ion Proton™ System)
  • Ion AmpliSeq™ Exome Kit
  • Ion TargetSeq™ Exome Enrichment : Ion-TargetSeq-Exome-50Mb-hg19

Experiment Work Flow

platform
protocol
Sample Type
최소 농도
최소 volume
QC포함 필요량
HiSeq
SureSelect exome
gDNA
25ng/ul(3ug) or 8.4ng/ul(1ug)
120ul
4ug or 2ug
HiSeq
Truseq exome
gDNA
20ng/ul
55ul
2ug
HiSeq
Nextera Rapid Capture Exome
gDNA
5ng/ul
10ul
500ng
HiSeq
Nextera Rapid Capture Expanded Exome
gDNA
5ng/ul
10ul
500ng
Proton
SureSelect(modify)
gDNA
8.4 ng/μL
120ul
2ug
Proton
LT exome
gDNA
8.4 ng/μL
120ul
2ug
Proton
AmpliSeq Exome
gDNA
4.8ng/ul
21ul
1ug

Turnaround time

platform
Illumina HiSeq Agilent SureSelect
Ion ProtonTM IonAmpliSeq Exome
Run Info
2x100 Paird End
Ion PITM Chip(v2)
실험 소요 기간 * raw data 생산 기준
6 weeks
2 weeks
* Raw data 생산 기준이며, 소요 기간은 샘플 수량에 따라서 조정될 수 있습니다.