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Service

This section provides basic information about Service

RNA Analysis

Overview

서로 다른 condition에서 RNA의 상대적 양변화를 측정하여 전체 genome 상의 모든 유전자 발현 정도를 동시에 확인할 수 있는 방법입니다. 특정 condition, chemical, 조직 등에서 발현이 증가 또는 감소되는 유전자를 선별해 낼 수 있습니다.
Affymetirx array는 3' IVT expression analysis, Whole-Transcript(WT) expression analysis, miRNA analysis로 구분됩니다. Bioanalyzer 2100, Nanodrop 1000을 이용한 철저한 quality control 과정 을 통해 신뢰도 높은 결과를 제공합니다.

Example for workflow & requirement

Products

Brand
Array type
Type
Array
Min. Conc. & Amount
Used Amt for Array Analysis
Affymetrix
3'IVT Expression
Eukaryotic organism
Human U133+, Primeview, Mouse 430, Rat 230, Arabidopsis, Barley, Bovine, C. elegans, Canine, Chicken, Citrus, Cotton, Drosophila, E. coli, Maize, Medicago, P. aeruginosa, Plasmodium/Anopheles, Poplar, Porcine, Rhesus Macaque, Rice, S.aureus, Soybean, Sugar Cane, Tomato, Vitis vinifera (Grape), Wheat, Xenopus laevis, Xenopus tropicalis, Yeast, Zebrafish 
130ng/ul, 10ul
390ng
Prokaryotic organism
650ng/ul, 25ul
11ug
Whole-Transcript(WT) expression
Human, Mouse, Rat, Human Exon, Mouse Exon, Rat Exon, Arabidopsis, Bovine, Canine, C. elegans, Chicken, Cyno-Rhes, Cynomolgus, Drosophila, Equine, Feline, Marmoset, Medicago, Ovine, Porcine, Rabbit, Rhesus, Rice (Cn), Rice (Jp), Rice (US), Soy Bean, Tomato, Zebra Finch, Zebrafish
100ng/ul, 10ul
300ng
miRNA(FlashTaq) Expression
miRNA 3.0, miRNA 4.0
200ng/ul, 15ul
1.6ug
Agilent
gene Expression
Arabidopsis (V4), Barley, Bovine (V2), Brassica, Canine (V2), C. elegans (V2), Chicken (V2), Cotton, Drosophila, E. coli, Horse, Magnaporthe (V2), Medicago, Mosquito, Porcine (V2), Rhesus Macaque (V2), Rabbit, Rice, Salmon, Sheep, Tobacco, Tomato, Wheat, Xenopus (V2), Yeast (V2), Zebrafish (V3)
100ng/ul, 10ul
300ng
miRNA
Human, Mouse, Rat
100ng/ul, 10ul
200ng
  • Benefit
  • - 유전자, 전사체 및 miRNA의 최신의 data까지 포함하고 있어 포괄적인 연구가 가능함
    - 다양한 포맷의 고객 맞춤형 content를 제공하므로 유연한 실험 디자인이 가능함
    - Affymetirx array의 경우 cartridge 형태로 샘플 수량에 상관없이 원하는 개수로 분석 가능

  • Workflow
  • - biotin labeled cRNA 합성
    - cRNA fragmentation
    - expression array hybridization
    - * Genechip Fluidics Station 450을 이용한 washing, staining
    - Scanning through Genechip Array scanner 3000 7G
    - Data Analysis

  • Sample requirement
  • RNA purity - 260/280 :1.8~2.1, 260/230 :1.8~2.1
    RNA integrity - rRNA [28s / 18s] Ratio of over 1.5 , RIN value of over 8

  • Turnaround timew
  • 샘플 QC 후 3주일 이내( 샘플 수와 array 종류에 따라 변경 가능 ) 분석 결과 제공



3' IVT Expression Analysis

Build on the knowledge of thousands of publications with 3' IVT solutions–the industry-standard tool for gene expression research.

Gene Regulation Analysis

GeneChip® Tiling Arrays are the most comprehensive array tools available for discovering novel RNA transcripts or mapping sites of protein/DNA interaction in chromatin immunoprecipitation experiments.

miRNA Analysis

Affymetrix' miRNA Arrays offer the most comprehensive coverage of miRNAs on a single array. Combined with Affymetrix® Expression Console™ Software and FlashTag™ Labeling Kits, the complete miRNA solution is a powerful tool for exploring the role of miRNAs.

Arrays

Cartridges

Human, Mouse, Rat, Other

Strips

Human, Mouse, Rat, Other

Plates

Human, Mouse, Rat, Other

Whole-Transcript Expression Analysis & Profiling

Get the most complete view of the genome with our line of whole-transcript expression products.

Arrays