This section provides basic information about Service

Isoform Sequencing
(Full-length Transcriptome Analysis)

RNA sequencing (RNA-Seq) by short read is widely used for gene expression and isoform analysis. However, it is difficult to analyze full-length transcripts with short reads due to alternative splicing events and transcriptional regulation. With Pacbio’s SMRT sequencing technology, we can sequence the entire transcriptome from 5’ end to 3’ polyA-tail without assembly

  • Discover novel transcripts & genes
  • Identify fusion genes
  • Resolve alternative polyadenylation
  • Identify retained introns
  • Find anti-sense transcription
  • Annotate gene isoforms & alternative splicing events
  • Recover missing exons
  • Improve isoform-abundance quantification accuracy